Quantification & standard curves
Mobiolink automatically detects standard samples within a run, computes the regression line and quantifies unknown samples. Validated curves can be stored as external references, after which a single anchor sample lets Mobiolink recalibrate the Y-intercept automatically in subsequent runs, even across changes in batches or instruments.
Inline standard curves (automatic detection)
Mobiolink bridges the gap between raw outputs (Ct values) and absolute quantitative results (copy numbers). When standard samples are placed directly on the PCR plate, the system recognizes them by the names defined in the assay master data, instantly computes the regression line (Ct value vs. concentration) and quantifies every sample marked 'Unknown' in the same run, with no manual mapping required.
External standard curves with anchor recalibration
Running a full set of standards on every plate is costly and often unnecessary thanks to modern cycler stability. A validated standard curve is saved as a reference; in subsequent runs you only include a single anchor sample with a known concentration. Mobiolink detects the anchor, calculates its deviation and automatically shifts the Y-intercept of the stored regression formula so the anchor sits exactly on the line. This allows precise quantification of unknown samples without spending valuable plate space on full calibration curves. While workflows in analytical chemistry often require continuous inline curves, Mobiolink lets molecular biology labs choose the most cost-effective and automated approach.