Extraction (RNA/DNA)
This stage systematically moves individual samples and quality controls into batch-based analysis. Extraction runs manually or automatically through sequential, batch-synchronized process units — through to a measurement-ready eluate.
Structured batch generation and control spiking
Mobiolink manages the assembly of the extraction plate or extraction sequence based on predefined templates. Individual primary samples are precisely combined with all mandatory control samples, standards, blanks, and quality controls into a unified batch. Immediately before the start of the actual extraction, the system supports the precise addition of internal controls (for PCR assays) or internal standards (for analytical chemistry) to each individual sample to ensure seamless analytical monitoring.
Cycle of sequential process units
The subsequent extraction process – whether performed manually or managed via automated liquid handling systems – is structured into a sequence of predefined, cyclic process units. The system guides and logs each sub-step, which is executed batch-wise and sequentially across all samples within the run:
- Precise addition of specific reagents and buffer solutions
- Temperature-controlled incubation phases
- Mechanical shaking and mixing workflows
- Selective washing steps for purification
- Centrifugation or separation cycles
Provision of measurement-ready eluates
The ultimate output of this coordinated batch run is a homogeneous, purified eluate. System-wide tracking of reagent lots, volumes, and operational steps guarantees that nucleic acids or target analytes remain stable. The resulting eluates are thus flawlessly prepared for the immediate subsequent measurements and the respective molecular or chemical detection techniques.