Mobiolink
  1. Phase 1 LIS Requests received
  2. 01

    Intake & pre-sorting

    • Systematic processing and classification
    • Intelligent error and exception management
    • Guided pre-processing
    • Dynamic sorting logic and workflow optimization

    Clear steps – less manual work

  3. 02

    Extraction (RNA/DNA)

    • Structured batch generation and control spiking
    • Cycle of sequential process units
    • Provision of measurement-ready eluates

    Secure extraction – fully documented

  4. 03

    PCR setup (plate layout)

    • Strategic consolidation and flexibility (n:n logic)
    • Digital safeguarding and rule verification prior to execution
    • The core USP for PCR laboratories
    • Generation of precise physical operational instructions

    Flexible and controlled – optimal allocation

  5. 04

    Measurement (analyzer)

    • Seamless data import at the analytical device
    • Autonomous execution and optional real-time tracking
    • Structured data export as a trigger for downstream stages

    Clear processes – reliable results

  6. 05

    Automated result interpretation

    • Background ingestion and precise sample mapping
    • Algorithmic curve evaluation and Ct value calculation
    • Comprehensive technical validation
    • Automated release and proactive flagging

    Reliable interpretation – fast and consistent

  7. 06

    Human validation

    • Efficient release matrix and targeted expert focus
    • Interactive curve analysis within the reporting dashboard
    • Complete documentation and audit trail
    • Fully automated LIS ingestion

    Technical validation – focus on what matters

  8. 07

    Export of results to the LIS

    • Quality-driven export barrier and authorization concept
    • Intelligent method mapping and multi-LIS routing
    • Automated code conversion
    • Process transformation in daily laboratory operations

    Results correctly assigned

  9. Phase 2 LIS Validated results exported
Communication with the LIS Process within Mobiolink
05

Automated result interpretation

This stage automates the primary evaluation and the technical quality control. Mobiolink imports the raw data, calculates the results and checks every control — only runs that pass all criteria are prepared for release.

Background ingestion and precise sample mapping

Mobiolink continuously monitors the designated target directories of the analytical instruments. As soon as an exported file from Stage 4 becomes available, the system ingests the raw data (curve kinetics, fluorescence signals, or raw values) fully automatically. Utilizing the allocation matrix documented in Stage 3, the software flawlessly maps each measurement result back to its corresponding primary sample and the administrative patient data from the LIS, completely eliminating manual transcription efforts.

Algorithmic curve evaluation and Ct value calculation

Following data ingestion, Mobiolink applies standardized mathematical evaluation algorithms to the raw data. The system analyzes the kinetics of the measurement curves, calculates the exact Ct (threshold cycle) values or concentrations, and performs the primary interpretation (positive, negative, equivocal). The parameters for this process are stored centrally and securely within the assay master data, guaranteeing absolutely reproducible results.

Mobiolink interface: plate view of a PCR run with sample status and QC

Comprehensive technical validation

Before any individual result is validated, the entire measurement series undergoes an automated technical review. Mobiolink validates the run performance systematically by verifying:

  • The presence and correct signal intensity of the internal controls (ICs) within each individual well.
  • The evaluation of global control references (positive controls, negative controls, blanks) to authorize the overall run validity.
  • The cross-checking of replicates and standard curves for mathematical plausibility and defined tolerance thresholds.

Automated release and proactive flagging

Samples that have successfully passed all technical controls and display clear measurement signals are automatically cleared by the system for final medical review and subsequent LIS transmission. However, if the system detects a breached quality control rule, an insufficient internal control signal, or an atypical curve trajectory, the affected sample is restricted. It is assigned a specific technical “flag” (warning label) and automatically routed to the laboratory supervisor for targeted manual inspection.

Next Human validation